Frequently Asked Questions about Feed Plate®

What does the Feed Plate® do?
Feed Plate® enables simulation of fed-batch in small scale. You will be able to precisely predict what your clones will do when fed-batch is applied.
What does Feed Plate® consist of?
Feed Plate® consists of a standard microplate where a special polymer matrix is immobilized at the bottom of each well. The matrix incorporates the substrate.
How does the Feed Plate® work?
Feed Plate® is a polymer based release system. After applying cultivation media into the well, the diffusion driven nutrient release starts in a defined, consistent release rate for up to 7 days cultivation time.
Do I need special media to work with Feed Plate®?
No, you can use your standard media. Feed Plate® is a physicochemical system and does not need any enzymes or special media or additives. The release kinetic is mainly dependent from cultivation temperature and media osmolarity. Therefore we refer to our cultivation conditions in the product catalogue.
How many samples can I use with Feed Plate®?
Feed Plate® is currently available in 24 and 96 well format what offers you the chance to handle many samples reliably in parallel. This allows simple and fast high throughput screenings.
Which format does the Feed Plate® have?
Feed Plate® is available in standard microplates. You choose which format you want to use. Special formats upon request.
I only use microplates from a specific supplier
No problem, we can apply our polymer to virtually any type of microplates (Cell culture tubes, 6-, 12- 24-, 48 or 96-well), any plate material (PP, PS, etc.) from any supplier (upon request).
Are other carbon sources or substrates available?
Besides standard carbon sources such as glucose, lactose or fructose also complex media (e.g. yeast extract) are possible. In addition Feed Plate® with nitrogen or phosphate sources are possible.
How about oxygen / I need a high oxygen transfer rate? May I still work in a 96 deep well plate?
Yes, no problem. Usually as long as you’re cultivating in fed-batch mode the oxygen supply is not an issue. In case you have questions about reaching a certain OTR we are happy to help with literature and advises.
How to store the Feed Plate®?
You can store the Feed Plate® at room temperature. Keep them dry and avoid direct sunlight.
How about the stability of the Feed Plate®?
Since the substrates incorporated in our matrix usually are stable crystalline substances, the stability of the Feed Plate® is more related to the sterility. Feed Plate® is beta-sterilised and stable for minimum two years.
Do you have a question, we have not answered on this page?
Please contact us: Call us or write us an email. We will answer any of your question.

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